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. 2015 Apr 6;66(11):3339–3352. doi: 10.1093/jxb/erv142

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© The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 5. — Free radical-scavenging ability and level of soluble proline in the wild type (WT) and JA biosynthesis mutants. (A) DPPH free radical-scavenging activity of standard butylated hydroxyanisole (BHA) and methanolic extract of WT and JA biosynthesis mutants under salinity stress of 100mM NaCl for 3 d. (B) IC₅₀ values were calculated from (A) depending on regression analysis. (C) Soluble proline in the WT, cpm2, and hebiba under salt stress. Proline was not detectable in control samples of the same genotypes. Values represent the mean of at least three independent experiments ±SE. Results for the WT, cpm2, and hebiba are indicated by white, grey, and striped bars, respectively. The dotted symbols in (A) and (B) represent the measurement for BHA. Significant differences amongst different treatments or genotypes are indicated by different letters, according to Tukey’s Honestly Significant Difference (HSD) test (P<0.05).