Table 2.
qRT-PCR expression analysis of barley antioxidant and defence-related transcripts in selected lesion mimic mutants in cv. Steptoe
| Steptoe | FN085 (nec1 ) | FN303 (nec8 ) | FN227 (nec9 ) | FN364 (nec9 ) | FN450 (nec9 ) | |
|---|---|---|---|---|---|---|
| Antioxidants | ||||||
| Ascorbate peroxidase 1 (APX1) |
1.000 (±0.129) |
0.486 (±0.104) |
0.405
(±0.075) |
0.802 (±0.174) |
0.584 (±0.084) |
0.544 (±0.093) |
| Ascorbate peroxidase 2 (APX2) |
1.000 (±0.245) |
0.543 (±0.123) |
0.656 (±0.110) |
1.270 (±0.588) |
0.580 (±0.147) |
0.398
(±0.077) |
| Catalase 1 (CAT1) |
1.000 (±0.348) |
1.369 (±0.518) |
1.745 (±0.516) |
1.208 (±0.427) |
1.598 (±0.505) |
1.901 (±0.392) |
| Catalase 2 (CAT2) |
1.000 (±0.386) |
1.906 (±0.693) |
1.960 (±0.402) |
1.156 (±0.342) |
1.428 (±0.467) |
0.403
(±0.079) |
| Glutathionine peroxidase 1 (GPX1) |
1.000 (±0.320) |
0.941 (±0.280) |
1.240 (±0.210) |
1.208 (±0.252) |
1.427 (±0.285) |
1.463 (±0.346) |
| Glutathionine peroxidase 2 (GPX2) |
1.000 (±0.126) |
0.498
(0.123) |
0.370
(±0.068) |
0.244
(±0.056) |
0.229
(±0.048) |
0.066
(±0.008) |
| Copper/Zinc superoxide dismutase 1 (CSD1) |
1.000 (±0.125) |
1.595 (±0.455) |
1.38 (±0.221) |
1.05 (±0.088) |
1.432 (±0.246) |
1.269 (±0.198) |
| Glutathione reductase 1 (GR1) |
1.000 (±0.058) |
0.524 (±0.145) |
0.672 (±0.142) |
0.471
(±0.064) |
0.434
(±0.076) |
0.420
(±0.060) |
| Defence-related | ||||||
| Pathogenesis-related 1 (PR1) |
1.000 (±0.245) |
125.217
(±35.130) |
94.328
(±15.461) |
51.110
(±13.154) |
53.724
(±23.629) |
39.386
(±7.111) |
| BAX-inhibitor 1 (BI-1) |
1.000 (±0.379) |
1.549 (±0.429) |
3.011
(±0.480) |
0.881 (±0.194) |
0.925 (±0.199) |
0.571 (±0.089) |
Mean normalised expression values are rescaled relative to Steptoe to show fold-change differences in transcript levels in mutant leaves compared to wild-type: >2-fold repressed, italics; >2-fold induced, bold (standard error shown in parentheses).