Table 1.
Statistical assessment of the amplification performance of 11 candidate genes in bovine blood mononuclear cells
| Gene* | Slope | StdErr s | P value s | Intercept | StdErr i | P value i | R 2 | E |
|---|---|---|---|---|---|---|---|---|
| 18s | −5.06151 | 0.0758 | 7.586E-10 | 25.3305 | 0.14632 | 2.507E-12 | 0.99866 | 0.57605 |
| 24s | −3.79032 | 0.05825 | 8.863E-10 | 31.739 | 0.11246 | 1.335E-13 | 0.99858 | 0.83582 |
| ACTB | −3.368 | 0.064719 | 3.379E-09 | 19.8495 | 0.12108 | 3.475E-12 | 0.99779 | 0.98113 |
| B2M | −3.93206 | 0.01988 | 1.127E-12 | 31.9222 | 0.03838 | 2.038E-16 | 0.99985 | 0.79606 |
| CTBP1 | −3.28926 | 0.09434 | 3.7E-08 | 35.1283 | 0.18211 | 1.31E-12 | 0.99509 | 0.98619 |
| GAPDH | −3.46422 | 0.03558 | 7.917E-11 | 31.7187 | 0.0687 | 6.966E-15 | 0.99937 | 0.94386 |
| MDM4 | −3.73883 | 0.0703 | 3E-09 | 38.6855 | 0.13572 | 1.258E-13 | 0.99788 | 0.85124 |
| PPIA | −4.31421 | 0.027696 | 4.722E-12 | 35.6981 | 0.058222 | 1.27E-15 | 0.99975 | 0.70527 |
| RAD50 | −3.94515 | 0.1124 | 4E-09 | 39.6633 | 0.20941 | 3.018E-14 | 0.99435 | 0.79257 |
| SDHA | −3.69503 | 0.1683 | 1.03E-07 | 36.2313 | 0.30674 | 8.221E-13 | 0.98569 | 0.86481 |
| YWHAZ | −3.55114 | 0.03416 | 5.343E-11 | 32.1879 | 0.06595 | 4.993E-15 | 0.99945 | 0.91249 |
Slope: Slope of standard curve; StdErrs: Standard error of the slope; P_values: Statistic significance of the slope estimation; Intercept: Intercept of standard curve; StdErri: Standard error of the intercept; P valuei: Statistic significance of the intercept estimation; R2: the correlation coefficient; E: Efficiency of amplification. * 18s: 18S ribosomal RNA; 24s: Ribosomal protein S24; ACTB: Actin, beta; B2M: Beta-2-microglobulin; CTBP1: C-terminal binding protein 1; GAPDH: Glyceraldehydes-3-phosphate dehydrogenase; MDM4: mouse double minute 4 protein; PPIA: Peptidylprolyl isomerase A; RAD50: RAD50 homolog (S. cerevisiae); SDHA: Succinate dehydrogenase complex, subunit A; YWHAZ: Tyrosine 3-monooxygenase/tryptophan 5-monoxygenase activation protein, zeta polypeptide.