Table 2.
Probabilities of significance of fixed effects on the expression of candidate housekeeping genes in bovine blood mononuclear cells under different in-vitro treatment. Significant probabilities (P ≤ 0.05) are shown in bold
| Candidate gene symbol* | 24s | CTBP1 | MDM4 | PPIA | RAD50 | |
|---|---|---|---|---|---|---|
| Source of variation | ||||||
| Mitogenic treatment (ConA) | ||||||
| Immunological Status# | 0.2936 | 0.8759 | 0.5817 | 0.1220 | 0.9072 | |
| Culture time§ | 0.2345 | 0.0041 | 0.0213 | 0.0002 | <.0001 | |
| Immunological Status*Culture time | 0.4287 | 0.4041 | 0.0036 | 0.5697 | 0.019 | |
| Antigenic treatment | ||||||
| Complex antigen (C. albicans) | ||||||
| Immunological Status# | 0.3501 | 0.6967 | 0.0032 | 0.1602 | 0.0524 | |
| Culture time§ | 0.0420 | 0.0179 | 0.3493 | 0.0007 | 0.0009 | |
| Immunological Status *Culture time | 0.4111 | 0.6621 | 0.0161 | 0.0148 | 0.8384 | |
| Single polypeptide (HEWL) | ||||||
| Immunological Status# | 0.0578 | 0.3333 | 0.0417 | 0.1948 | 0.0415 | |
| Culture time§ | 0.0970 | 0.1510 | 0.9049 | 0.0055 | 0.6798 | |
| Immunological Status *Culture time | 0.2852 | 0.2769 | 0.2026 | 0.8707 | 0.6076 | |
*24s: Ribosomal protein S24; CTBP1: C-terminal binding protein 1; MDM4: mouse double minute 4 protein; PPIA: Peptidylprolyl isomerase A; RAD50: RAD50 homolog (S. cerevisiae). # General effect of immunological status includes naïve, immunized, and boosted groups. § General effect of culturing time included 4 and 24 hour time points. Bonferroni correction was not applied to this analysis since multiple comparison was not appropriate for all the fixed effect in this model