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. Author manuscript; available in PMC: 2015 May 30.
Published in final edited form as: Science. 2014 Aug 28;346(6205):98–101. doi: 10.1126/science.1254536

Fig 2. TRM reactivation induces DC maturation.

Fig 2

CD86 and CCR7 expression was evaluated on CD11c+/MHC-II+ DC in the FRT 12h after t.c challenge of (A) P14 immune chimeras challenged with gp33 peptide, (B) LCMV immune mice (that never received P14 cells) challenged with gp33 peptide, (C) P14 immune chimeras that were t.c. challenged with either VV-gp33 or VV-OVA, or (D) P14 immune chimeras that were injected i.p. with 1 μg of anti-Thy1.1 antibody five days prior to gp33 challenge. (E) Intracellular TNFα expression was evaluated in P14 CD8 T cells from the FRT by flow cytometry 12h after t.c. gp33 challenge. (F&G) DC phenotype was evaluated as in (A), but the indicated mice were pretreated with TNFα blocking antibody. Representative of 2-3 experiments totaling 6-14 mice/group. *=p<0.05, **=p<0.01, ***=p<.001, unpaired two-tailed t-test, error bars indicated mean±SEM.