FIG. 2.

Survival of explanted human and rat neural retina. Sections through neural retina cocultured with different sources of RPE (no RPE, RPE-J, ARPE19, or human ESC-RPE) at 1 week (A; n=2) and 2 weeks (B; n=2). Blue fluorescent signal highlights the different nuclear layers of the retina in comparison to a fresh tissue section (reference); GCL, ganglion cell layer; INL, inner nuclear layer; ONL, photoreceptor outer nuclear layer. Scale bar=40 μm. The grayscale sections below each immunofluorescent image are high-resolution TUNEL images of the ONL of the coculture above it. Scale bar=50 μm. All cocultures are with rat neural tissue except for the panels labeled as human sections. Color images available online at www.liebertpub.com/tea