FIG. 3.

Angiogenesis occurs in proximal regions of the graft. (A) Eight-week post-transplant bladder stained for GFP (green), CD31 (red), and nuclei (DAPI, blue); 10× magnification. White lines identify the anastomotic regions. (B) Representative images of proximal, middle, and distal areas from 8-week graft; 60× magnification. (C) Graphical representation of the GFP index (geometric mean) at each of the three regions from animals at 2, 4, 8, and 16 weeks post-transplant. Statistically significant differences are indicated by asterisks (*). When adjusted for time point, the GFP index of P>D (p<0.001) and P>M (p=0.002) in any given animal at any time point. Color images available online at www.liebertpub.com/tea