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. 2015 Apr 22;21(11-12):1906–1915. doi: 10.1089/ten.tea.2014.0630

FIG. 4.

FIG. 4.

Perfusion in distal regions of bladder grafts occurs primarily through wild-type donor blood vessels. (A, B) Representative distal fields from 1- to 8-week animals show the origin of perfused vessels. Perfused microspheres (blue) are found in CD31+ (red) vessels, which are either GFP+ (host, green; arrow heads) or GFP− (wild-type donor; asterisk); 60× magnification. (C) Representative proximal field from 8-week animals shows both perfused GFP+ and GFP− blood vessels; 60× magnification. (D) Graphical representations of the relative percentage of GFP+ (host) versus wild-type (GFP−, donor) blood vessels in distal areas from 1-, 8-, and 16-week animals. (E) Proximal areas from 2-week transplant bladders were stained for CD31 (red), GFP (green), and nuclei (blue, DAPI) and analyzed for host (GFP+) or donor (wild type, GFP−) cells within blood vessels. Possible GFP-WT chimeric blood vessels are indicated via red and green arrows, pointing to areas within a vessel that are GFP− (donor, red only) and GFP+ (host, yellow-orange), respectively. GFP host vessels are shown as a positive control for GFP+ blood vessels (yellow-orange); 60× magnification. Color images available online at www.liebertpub.com/tea