FIG. 5.

Limited vasculogenesis in an intact bladder wall transplant. Immunohistochemical analysis of bladder tissues from control animals (A, B) or bladder transplant recipients (C–L). (A) LacZ is highly expressed in LacZ transgenic tissues. Asterisk (*) denotes LacZ⁺ vessel. LacZ as green, CD31 as brown; 20× (left) and 60× (right) magnifications. (B) LacZ is not expressed in GFP/LacZ chimeric host bladder tissue pretransplant (left; 20× magnification) and only minimally after transplant (right; 40× magnification). Asterisk (*) denotes LacZ⁺ cells. (C–G) One-week post-transplant bladder. (C) LacZ/CD31 staining of the graft; 10× magnification. Lines demarcate the anastomoses. (D) LacZ staining correlates with inflammatory infiltrates, as seen by H&E (E); 10× magnification. Arrowheads indicate sutures in the anastomotic region. (F, G) LacZ staining is found within vessels, but not as a part of the vessels; H&E (F) and LacZ/CD31 (G); 40× magnification. (H–L) Eight-week post-transplant bladder. (H) LacZ staining of the graft; 10× magnification. Lines demarcate the anastomoses. LacZ staining in (I) correlates with inflammatory infiltrates, as seen by H&E in (J); 40 × magnification. (K) Immunofluorescent stain demonstrates a small percentage of GFP⁺ infiltrates in the same inflammatory area captured in (I, J) (GFP, green; CD31, red; nuclei-DAPI, blue); 60× magnification. (L) LacZ⁺ cells observed around a vessel; 40× magnification. Color images available online at www.liebertpub.com/tea