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. 2015 Mar 24;21(11-12):1772–1784. doi: 10.1089/ten.tea.2014.0671

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© R.M. Gouveia et al. 2015; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons Attribution Noncommercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.

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FIG. 8. — Immunofluorescence confocal microscopy of self-detached tissue constructs. (a) Representative top view and (b) z-stack view of corneal tissues fabricated by hCSFs grown on _mfPA:_dPA templates for 90 days. Tissue constructs showed that they comprise multiple layers of collagen type I fibers (red) deposited between actin-stained cells (green) and form an ECM enriched with hCSF-characteristic proteoglycans (blue), such as keratocan (left), lumican (center), and decorin (right panels). Scale bars, 20 μm. Color images available online at www.liebertpub.com/tea