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. 2015 Mar 24;21(11-12):1772–1784. doi: 10.1089/ten.tea.2014.0671

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© R.M. Gouveia et al. 2015; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons Attribution Noncommercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.

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FIG. 9. — Photograph of free-floating, human corneal tissue fabricated by hSCFs. Tissue constructs fabricated by cells during 90 days recovered their ability to express MMPs after being maintained in SFM without RA. The endogenous MMPs expressed by the tissue were capable of degrading the underlying _mfPA:_dPA template after 3 days, thus inducing the tissue self-release from its adhesive bottom surface without any external manipulation. The resulting free-floating tissue was transparent in water and retained its original shape and structural integrity. A decimal ruler was used as scale.