FIG. 6.

Conditioned neural stem/progenitor cell (NSPC) media (NSCM) enhances cortical astrocyte proliferation and reduces glial fibrillary acidic protein (GFAP) expression after scratch wound injury in culture. Photomicrographs show typical astrocytes observed at the edge of the scratch wound stained with Ki-67, nestin, and GFAP after being cultured for 48 h in unconditioned media (A–D) or undiluted NSCM (E–H). Proliferating Ki-67–positive astrocytes predominantly co-label with nestin and not GFAP. (J) Graph represents increased Ki-67 staining of astrocytes at the edge of the scratch wound after being cultured with NSCM or centrifuged NSCM for 48 h. (K) Graph shows reduced GFAP expression of astrocytes at the edge of the scratch wound after being cultured with NSCM or centrifuged NSCM for 48 h. n=3 independent experiments in triplicate per group. Scale bar=200 μm. *p<0.05, **p<0.01, **p<0.001. Color image is available online at www.liebertpub.com/neu