FIG. 2.

Live/Dead analysis of transfected hWJCs. Transfection with HATH1 led to greater cell viability than transfection with MATH-1. A minimum of 20,000 events were analyzed through flow cytometry to determine the viability of treatments 1 day after transfection. The top row shows the distribution of cells from forward scatter. The middle row displays cells with an intact nucleus positively identified by Hoechst staining. The bottom row displays the distribution of cells identified as live or dead based on Sytox Red (dead cell stain) staining. The histograms shown are an arbitrary selection from one umbilical cord of five tested (n=5). MATH-1 represents cells transfected with MATH-1 pDNA. HATH1 represents cells transfected with HATH1 pDNA. H1/H5 represents cells transfected with HES1 and HES5 siRNA. MATH-1/H1/H5 represents cells cotransfected with MATH-1 pDNA, HES1 siRNA, and HES5 siRNA. HATH1/H1/H5 represents cells cotransfected with HATH1 pDNA, HES1 siRNA, and HES5 siRNA. hWJCs, human Wharton's jelly cells. Color images available online at www.liebertpub.com/tea