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. 2015 Apr 10;21(11-12):1795–1809. doi: 10.1089/ten.tea.2014.0340

FIG. 5.

FIG. 5.

Gene expression of transfected hWJCs. HATH1/H1/H5-transfected cells showed significant increases in gene expression across most genes compared with untreated control cells 1 day after transfection, whereas MATH-1-transfected cells only showed significant increases in gene expression in JAG2, HES1, and HES5. Treated hWJCs were assessed for gene expression using RT-qPCR at 1, 3, and 7 days after transfection. *Statistically significant difference from untreated hWJCs (p<0.05). ATOH1, HES1, and HES5 are genes pertaining to pDNA and siRNA used for different treatments. SOX2 initiates ATOH1 expression and is then downregulated by ATOH1. No significant differences were detected among groups regarding JAG1 expression. JAG1 and JAG2 are NOTCH ligands that participate in the NOTCH signaling pathway. MYO7A, GFI1, and POU4F3 are key hair cell markers. MYO7A is a critical marker that was significantly expressed in hWJCs treated with HATH1/H1/H5 at 1 and 3 days after transfection. The results are representative of cells collected from five different umbilical cords (n=5) and are reported as statistical means. All experiments were performed in triplicate. Error bars represent standard deviations. H1/H5 represents cells transfected with HES1 and HES5 siRNA. MATH-1/H1/H5 represents cells cotransfected with MATH-1 pDNA, HES1 siRNA, and HES5 siRNA. HATH1/H1/H5 represents cells cotransfected with HATH1 pDNA, HES1 siRNA, and HES5 siRNA. ATOH1, atonal homolog 1.