FIG. 6.

Immunostaining of transfected hWJCs. HATH1/H1/H5-transfected cells showed increased expression of MYO7A and HES5 from 1 to 7 days after transfection, whereas MATH-1-transfected cells showed a decrease in MYO7A and HES5 expression. Treated hWJCs were assessed for protein expression by immunostaining at 1 and 7 days after transfection. Primary antibodies were preconjugated to quantum dots. Cell nuclei are represented by Syto 9 staining (blue). HES1 (orange) was not positively identified. HES5 (yellow) and MYO7A (red) were positively identified in all treated groups. Cells transfected with HATH1/H1/H5 displayed positive identification of GFAP (pink) 1 day after transfection. H1/H5 represents cells transfected with HES1 and HES5 siRNA. The images shown are an arbitrary selection from one umbilical cord of five tested (n=5). MATH-1/H1/H5 represents cells cotransfected with MATH-1 pDNA, HES1 siRNA, and HES5 siRNA. HATH1/H1/H5 represents cells cotransfected with HATH1 pDNA, HES1 siRNA, and HES5 siRNA. Scale bar=20 μm. GFAP, glial fibrillary acidic protein. Color images available online at www.liebertpub.com/tea