FIG. 7.

Type II collagen (Col II) deposition (brown, here represented in darker shades of grey within pellets ECM) assessed by immunohistochemistry (IHC) of paraffin sections of MSC pellets cultured for (A) 14, and (B) 21 days in the three culture conditions tested: BM, CM, or CM supplemented with γ-PGA (CM+γ-PGA). One representative donor of each cell type is presented here. For each time point: the first column presents whole pellet images (bars represent 500 μm). The three columns to the right are detailed images of distinct regions of the cell pellet: peripheral, middle, and center of the pellets. The bars represent 50 μm. Black arrows point to fibrillar Col II. Color images available online at www.liebertpub.com/tea