FIG. 8.

Col II deposition (brown, here represented in darker shades of grey within pellets ECM) assessed by IHC of paraffin-sections of NC pellets cultured for (A) 14, and (B) 21 days in the three culture conditions tested: BM, CM or CM supplemented with γ-PGA (CM+γ-PGA). One representative donor of each cell type is presented here. For each time point: the first column presents whole pellet images (bars represent 500 μm). The three columns to the right are detailed images of distinct regions of the cell pellet: peripheral, middle, and center of the pellets. The bars represent 50 μm. Color images available online at www.liebertpub.com/tea