FIG. 1.

Ex vivo decellularization of whole rat livers. Macroscopic appearance of the liver during different phases of decellularization (A–C). Translucent structures were obtained at the end of the process (D) and it is possible to identify a network of tubular structures derived from the remnants of vessel and biliary duct systems. Representative micrographs of hematoxylin and eosin (H&E) staining of normal (E) and decellularized liver tissues (F) showing that after decellularization, a fine extracellular matrix (ECM) network was conserved, with the absence of cellular components. Scale bar=100 μm. Color images available online at www.liebertpub.com/tea