Skip to main content
. Author manuscript; available in PMC: 2015 May 30.
Published in final edited form as: Adv Immunol. 2014;124:1–16. doi: 10.1016/B978-0-12-800147-9.00001-7

Figure 1.

Figure 1

Lung ILC2s respond vigorously to IL-33 and produce a large quantity of IL-5 and IL-13 in vitro. (A) Gating strategy and identification of ILC2s in lung single cell suspensions from naïve BALB/c mice. (B) Four populations of lung cells, including Lin+ cells, Lin−CD25−CD44− cells, Lin−CD25−CD44+ cells and Lin−CD25+CD44hi cells (i.e. ILC2s) were isolated from naïve BALB/c mice by FACS sorting. Sorted and unsorted lung cells were cultured with medium alone or with IL-33, and the levels of cytokines in the supernatants were measured by ELISA. (C) Morphology of lung ILC2s. Lung ILC2s were cultured with medium alone or IL-33 and examined under electron microscopy. Original magnifications; 25,000x (medium alone, left) and 12,000x (IL-33, right).