Table 1.
Stable nitrogen isotopic composition (δ15N value) of amino acids in the three diets and in tadpoles before and after feedings
| Days after being fertilized | δ15N (‰, relative to Air) | |||||||
|---|---|---|---|---|---|---|---|---|
| Alanine | Glycine | Valine | Leucine | Isoleucine | Methionine | Glutamic acid | Phenylalanine | |
| Diets | ||||||||
| Commercial pellets | 15.9 | 4.4 | 15.3 | 13.1 | 11.7 | 3.0 | 13.4 | 7.3 |
| Bloodworms | 15.9 | 7.1 | 17.3 | 7.2 | 7.4 | 2.7 | 19.1 | 6.8 |
| Boiled rice | 4.7 | −0.1 | 3.8 | 2.4 | 0.7 | −3.7 | 2.8 | 10.1 |
| Tadpole before feeding | ||||||||
| 0 | 14.9 | 7.5 | 15.1 | 10.9 | 10.9 | 0.2 | 14.6 | 4.6 |
| 7 | 16.3 | 2.7 | 16.4 | 10.4 | 17.3 | 0.4 | 16.4 | 4.3 |
| 9 | 16.3 | 3.9 | 18.2 | 11.9 | 19.3 | 0.3 | 17.2 | 4.7 |
| Tadpole feeding on commercial pellets | ||||||||
| 11 | 21.5 | 8.7 | 18.9 | 13.6 | 19.5 | n.d. | 20.2 | 7.0 |
| 15 | 22.6 | 12.5 | 19.8 | 14.4 | 19.0 | 3.1 | 21.2 | 7.1 |
| 19 | 23.0 | 9.5 | 20.5 | 14.4 | 19.3 | n.d. | 22.2 | 7.8 |
| 23 #1 | 23.2 | 9.7 | 21.4 | 14.1 | 19.6 | 3.5 | 21.3 | 7.4 |
| 23 #2 | 23.3 | 8.4 | 21.9 | 15.7 | 18.9 | 2.3 | 22.1 | 7.3 |
| 27 #1 | 20.0 | 8.6 | 20.3 | 16.0 | 18.9 | 3.5 | 20.7 | 7.0 |
| 27 #2 | 24.0 | 11.9 | 21.9 | 14.7 | 18.1 | n.d. | 20.8 | 7.9 |
| 31 #1 | 23.9 | 12.5 | 21.1 | 15.1 | 19.3 | n.d. | 21.7 | 7.0 |
| 31 #2 | 23.8 | 10.6 | 22.6 | 15.8 | 20.1 | 4.1 | 21.1 | 7.9 |
| 35 #1 | 24.3 | 10.5 | 22.7 | 15.1 | 19.1 | 3.1 | 21.2 | 7.5 |
| 35 #2 | 23.6 | 9.7 | 22.7 | 15.0 | 19.5 | n.d. | 21.4 | 7.4 |
| 39 #1 | 23.7 | 11.4 | 23.4 | 14.8 | 19.5 | n.d. | 22.1 | 7.6 |
| 39 #2 | 23.4 | 9.8 | 22.5 | 14.7 | 19.5 | n.d. | 21.8 | 7.9 |
| Tadpole feeding on bloodworms | ||||||||
| 11 | 17.6 | 6.1 | 21.2 | 13.2 | 16.5 | n.d. | 25.6 | 6.8 |
| 15 | 18.6 | 8.4 | 22.2 | 13.9 | 15.9 | n.d. | 26.6 | 6.9 |
| 19 | 19.6 | 12.2 | 21.4 | 13.5 | 14.5 | n.d. | 26.7 | 7.0 |
| 23 #1 | 21.0 | 10.1 | 21.6 | 13.1 | 15.4 | 3.8 | 27.2 | 6.9 |
| 23 #2 | 21.3 | 9.7 | 21.9 | 13.4 | 14.6 | 3.8 | 26.9 | 7.4 |
| 27 #1 | 20.1 | 8.3 | 22.2 | 12.7 | 14.9 | n.d. | 26.5 | 7.1 |
| 27 #2 | 20.9 | 9.7 | 21.4 | 12.8 | 15.1 | n.d. | 27.4 | 6.7 |
| 31 #1 | 20.9 | 10.2 | 21.2 | 13.3 | 14.8 | 2.7 | 26.8 | 7.1 |
| 31 #2 | 20.2 | 11.4 | 22.2 | 13.1 | 14.5 | n.d. | 26.9 | 7.0 |
| 35 #1 | 21.2 | 9.1 | 21.9 | 12.3 | 13.5 | 2.4 | 27.2 | 7.2 |
| 35 #2 | 20.8 | 8.5 | 22.1 | 13.3 | 14.6 | n.d. | 26.8 | 7.3 |
| 39 #1 | 21.3 | 12.6 | 22.5 | 12.6 | 13.7 | n.d. | 26.9 | 7.3 |
| 39 #2 | 20.5 | 8.4 | 21.9 | 13.7 | 14.6 | 3.1 | 26.9 | 7.3 |
| Tadpole feeding on boiled rice | ||||||||
| 11 | 11.8 | 1.6 | 12.8 | 7.4 | 13.8 | −2.4 | 12.7 | 7.2 |
| 15 | 10.4 | 3.5 | 11.0 | 6.0 | 10.2 | n.d. | 9.5 | 9.0 |
| 19 | 9.7 | 1.6 | 9.7 | 5.6 | 8.7 | n.d. | 8.4 | 9.8 |
| 23 | 9.2 | 1.4 | 9.4 | 6.2 | 7.1 | n.d. | 5.3 | 9.5 |
| 27 | 9.6 | 0.3 | 9.4 | 5.0 | 6.3 | −3.3 | 4.0 | 10.6 |
| 31 | 8.8 | −0.2 | 8.3 | 4.5 | 6.4 | −3.9 | 4.8 | 9.9 |
| 35 | 9.0 | −0.2 | 7.0 | 5.6 | 5.1 | n.d. | 4.7 | 10.3 |
| 39 | 9.7 | 2.0 | 7.0 | 4.8 | 5.3 | n.d. | 4.1 | 10.8 |
| 43 #1 | 8.6 | 0.2 | 7.7 | 4.6 | 4.5 | −3.2 | 4.1 | 10.7 |
| 43 #2 | 8.5 | −1.4 | 7.3 | 4.4 | 5.5 | −3.7 | 3.5 | 10.7 |
| 47 #1 | 8.3 | −0.3 | 7.9 | 4.5 | 5.1 | n.d. | 4.1 | 10.5 |
| 47 #2 | 9.1 | −0.4 | 7.9 | 4.2 | 4.8 | −3.5 | 4.4 | 10.4 |
| 51 #1 | 8.6 | −1.0 | 7.1 | 4.9 | 4.6 | −3.4 | 3.5 | 11.0 |
| 51 #2 | 7.9 | −0.7 | 7.8 | 4.4 | 4.4 | −3.3 | 3.1 | 10.7 |
| 55 #1 | 7.5 | 0.2 | 7.1 | 4.7 | 4.2 | −3.2 | 3.7 | 10.9 |
| 55 #2 | 7.9 | −0.7 | 7.3 | 4.4 | 4.7 | −3.4 | 2.9 | 10.6 |
| 59 #1 | 7.9 | 0.7 | 7.3 | 3.9 | 4.6 | n.d. | 3.6 | 10.5 |
| 59 #2 | 8.1 | −0.5 | 7.1 | 4.0 | 4.9 | −3.0 | 3.2 | 10.7 |