Fig 2.

Difference in the phenotype of splenic B cells from male and female SWR × NZB F₁ (SNF₁) mice. (a) Spleen cells from male and female SNF₁ mice aged 4 and 16 weeks were examined for surface CD19 and IgG by fluorescence activated cell sorter (FACS). Shown are representative FACS plots for male and female groups of mice and mean ± standard error of the mean (s.e.m.) values. (b) Intracellular cytokine expression in B cells was examined by FACS. Splenocytes were activated with PMA/ionomycin and LPS in the presence of brefeldin A and the cells were stained for surface CD19 and intracellular cytokines. Representative FACS plots and mean ± s.e.m. values are shown.