Fig 3.

Recombinant Toxascaris leonine galectin (rTl-gal) increased the infiltration of CD45R/B220⁺ cells into the central nervous system (CNS) at chronic stage. The immunohistochemical images depicted CD3⁺ (a,d), CD45R/B220⁺ (b,e) and F4/80⁺ (c,f) cells in lumbar spinal cords of rTl-gal-treated and vehicle-treated experimental autoimmune encephalomyelitis (EAE) mice, respectively. Haematoxylin staining was performed to illustrate nuclei. Controls with secondary antibody alone were negative throughout (g–i). Quantification of CD3⁺ (j), CD45R/B220⁺ (k) and F4/80⁺ (l) infiltrated cells per high-power field (HPF, magnification × 400) was performed using three lesions showing the most representative expression from each mouse. Results are representative of more than three independent experiments. Bars = 60 µm.