Fig 4.

Recombinant Toxascaris leonine galectin (rTl-gal) increased astrogliosis in spinal cords at chronic stage. Spinal cord sections from vehicle- or rTl-gal-treated EAE mice were stained for glial fibrillary acidic protein (GFAP) (a, astrocytes) and Iba-1 (b, microglia). Quantification of fluorescence intensity demonstrates rTl-gal-treated mice had more GFAP reactivity than vehicle-treated mice. 4',6-diamidino-2-phenylindole dihydrochloride (DAPI) staining was used for indicating nuclei. Results are representative of more than three independent experiments. Values represent the mean ± standard error of the mean (s.e.m.). Statistical difference of P < 0·05 (*) for rTl-gal-treated mice versus controls is indicated. Bars = 60 µm.