Fig 4.

Membrane attack complex (MAC) deposition in relapsing–progressive experimental autoimmune encephalomyelitis (EAE). (a–i) Representative immunohistochemical stainings in spinal cord sections from naive and chronic relapsing experimental autoimmune encephalomyelitis (crEAE) mice, showing MAC staining in meninges and white matter during acute disease (e), whereas abundant MAC staining localizes in grey matter (i) during progressive disease. Spinal cords from naive mice were negative for MAC (a,d,g). (j) Quantification of MAC immunostaining in cross-sections of cervical, thoracic and lumbar spinal cord regions from naive mice and during acute, remitting, relapsing and progressive disease stages of crEAE. Values are expressed as mean ± standard deviation. The asterisks (*) indicate statistically significant differences determined by one-way analysis of variance (anova) (P ≤ 0·05).