Fig 6.
Verification of hepatitis C virus (HCV) infection of monocytes. (a) HCV infection of Huh7·5 cells with JFH-1T [multiplicity of infection (MOI) 0·1]. Cells were analysed by reverse transcription–polymerase chain reaction (RT–PCR). Negative-strand HCV RNA 2 days after infection (lane 1), 3 days after infection (lane 2) and 5 days after infection (lane 3). (b) HCV infection of monocytes 2 and 5 days after inoculation with JFH-1T (MOI 0·1). Positive-strand HCV RNA was analysed by RT–PCR 2 days after infection (lane 1) and 5 days after infection (lane 2) and negative-strand HCV RNA 2 days after infection (lane 3) and 5 days after infection (lane 4) in mature dendritic cells (DCs). (c) HCV NS5a RNA identified by RT–PCR in monocytes. (d) Western blot of NS5a expression in JFH-1T-infected monocytes after 2 (lane 1) and 5 (lane 2) days. (e) HCV-NS5a detected in JFH-1T-infected monocytes using fluorescent microscopy (×20). (f) HCV-NS5a in JFH-1T-infected Huh7·5 cells as positive control (×20).