Fig 5.

Citrate modulates global histone acetylation. Changes in histone H3 and H4 global acetylation were determined by Western blot in acid-extracted lysates of human acute monocytic leukaemia cell line (THP-1) cells. Cells were preincubated with varying concentrations of citrate (0, 1, 6 mM) prior to stimulation with lipopolysaccharide (LPS) (0–90 min) in (a) normal calcium (0·4 mM) or (b) supplemental calcium (1·4 mM) media. Relative band intensity is represented by the numbers below the Western blots with the no citrate time zero condition assigned a relative value of 100.