Fig 2.

Physical interaction between mitogen-activated protein kinase phosphatase-1 (MKP-1) and phosphorylated cytosolic phospholipase A2 (cPLA₂). (a–d) Lung and cell lysates were immunoprecipitated using anti-p-cPLA₂ and anti-MKP-1 antibodies. (e,f) Lung and cell lysates were immunoprecipitated using anti-cPLA₂ antibody. (g) Cells were probed with anti-phospho-cPLA₂ or anti-MKP-1 antibody and conjugated with oligonucleotide PLA probe: nucleus was counterstained with 4',6-diamidino-2-phenylindole (DAPI). A representative of two or three independent experiments with three mice/time-point/experiment is shown in (a–f). A representative of four independent experiments is shown in (g).