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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Pharm Res. 2015 Jan 16;32(7):2280–2291. doi: 10.1007/s11095-015-1619-0

Fig 5. The release of lysosomal enzyme, N-acetyl-β-D-glucosaminidase (NAG), mediated by blend particles.

Fig 5

DC2.4 cells were exposed to blend particles or PS particles for 4 h at 37°C, and then treated with digitonin. The fraction of permeabilized cells assessed by trypanblue staining for each cell types (blend0.1, blend0.2, blend0.5, PS-NH2, PS-COOH) (a). Black: permeabilized cells; white: membrane-intact cells. Cytosol NAG activity was measured with β-N-Acetylglucosaminidase assay kit and expressed as enzyme units per 105 permeabilized cells (b).