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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Kidney Int. 2015 Feb 4;87(6):1125–1140. doi: 10.1038/ki.2014.406

Figure 8. Dicer1 mutation disrupts WNT signaling in renal stromal cells.

Figure 8

(A) qPCR data showing changes in the expression of Wnt4, Wnt 11 and regulators of the canonical Wnt signaling, Axin2, Lef1 and Wisp1 in whole kidney. (B–D) Confocal images of LEF1 (B), β-catenin (C) and p57Kip2 (D) in developing renal medulla. Arrowheads indicate interstitial positively stained cells. Note that mutant stromal cells showed marked reduction in the expression of LEF1 and β-catenin accumulation as well as reduction in p57Kip2 at both E15.5 and P0. (E–G) Quantification of number of LEF1+ (E), nuclear β-catenin+ (F) and p57Kip2+ (G) per mm2 of kidney. Bar, 25 μm. **P < 0.01, n = 3–5/group.