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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Kidney Int. 2015 Feb 4;87(6):1125–1140. doi: 10.1038/ki.2014.406

Figure 9. Dicer1 mutation results in loss of stromal cell microRNA that serve to promote human renal stromal cell migration, proliferation and expression of factors.

Figure 9

(A) Heatmap showing hierarchical cluster analysis of significantly differentially regulated microRNA between control, Foxd1+/GC; Dicer1fl/fl, E15.5 and P0 kidneys. Mutant kidneys cluster together and show 46 miRNA with reduced expression compared with 4 which were increased. When these miRNA were interrogated against miRNA that are enriched in stromal cells 7 miRNA were identified (right panel). (B) Photomicrographs showing effect of anti-miR-214 and -199a-3p on migration. (C) Graph showing the effect of different anti-miRNAs on migration when stimulated by TGFβ after 24h. (D) Graph showing the effect of different anti-miRNAs on proliferation when stimulated by a combination of PDGF-AA and -BB after 16h. (E) Images of phalloidin-Cy3 stained stromal cells showing the formation of lamellipodia and filopodia in response to TGFβ at 24h (arrowheads), a response markedly diminished following silencing of miR-214 or miR-199-3p (F) Graphs showing the effect of anti-miR treatment on transcript levels. Bar, 25 μm. *P < 0.05, **P < 0.01. n = 3–5/group