A) Differential expression of transcripts based on RNA-sequencing data from anterior and posterior embryo halves. Red, score > 2.5 and p < 0.05. Blue, putative germ cell/plasm components. B) RNA in situ hybridizations of early embryos for panish. Anterior is left; scale bars, 10 μm. C) C-clamp region of panish aligned with Pangolin sequences from C. riparius (Cri), D. melanogaster (Dme), Anopheles gambiae (Aga), and Tribolium castaneum (Tca). Gray numbers, residues not shown; red, conserved cysteine residues; blue, residue similarity. D) The panish locus. Homology based on reciprocal-best-BLAST with D. melanogaster. Longest ORFs (shaded) and orientation (arrow heads) are indicated.