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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Apoptosis. 2015 Jul;20(7):996–1015. doi: 10.1007/s10495-015-1131-3

Fig. 3. Receptor expression and spheroids formation in WM35 and WM9 melanoma cell lines.

Fig. 3

(A and B) Surface and total expression levels of NGFR, TNFR1, FAS and CD133 in WM35 and WM9 human melanoma lines. % Antigen-positive cells are indicated. (C) Spheroid formation by WM35 cells in serum-free media supplemented with FGF2 (20 ng/ml), EGF (20 ng/ml) and insulin (5 μg/ml). Phase-contrast images are shown. (D) NSC (control) and WM9 melanoma cells after 6 days of incubation in the similar serum-free media with supplements. (E) Surface expression of indicated receptors on WM35 cells after cell culture growth in two types of media. (F) Apoptotic levels after treatment of WM35 cells that were grown on the complete (WM35) or serum-free media with supplements (WM35-SF). Apoptosis was induced by combination of TRAL (50 ng/ml) + CHX (1 μg/ml) or TNFα (20 ng/ml) + CHX. Levels of apoptosis were determined using PI staining and cell cycle-apoptotic analysis. Error bars represent mean ± SD (p < 0.05, Student’s t test). Stars indicate significant difference.