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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Apoptosis. 2015 Jul;20(7):996–1015. doi: 10.1007/s10495-015-1131-3

Fig. 9. (A) Neuronal differentiation of NSC (10 days) in serum-free differentiation media supplemented with B27 that contains insulin (5 μg/ml). (B and C) Neuronal pathway of differentiation of 1205Lu and OM431 melanoma cells (10 days) in the differentiatione media with the B27 supplement.

Fig. 9

Confocal images were developed after immunostaining using rabbit polyclonal Ab to SOX2, a pluriopotency marker (green) and monoclonal antibody to Nestin, an early neuroprogenitor marker; polyclonal Ab to Doublecortin, a neuronal marker (green) and monoclonal Ab to Nestin (red); polyclonal Ab to phospho-ERK (green) and PI, polyclonal Ab to STAT3 (green) and PI (red). Bar = 50 μm. Images from independent experiments: (I), (Ia) and (Ib); (IIIa), (IIIb) and (IIIc). (D) 1205Lu cells, which were stably transfected with dominant-negative Rac1N17, after 10 days in differentiation media. (E and F) Relative length of Nestin-containing neurites before (day 0) and 10 days after differentiation was determined using 5 random areas of images. Error bars represent mean ± SD (p < 0.05, Student’s t test). Stars indicate significant difference in relative neurite length for normal 1205Lu cells at days 0 and 10; double star indicates significant difference between control and Rac1N17-transfected 1205Lu cells at day 10.