Table 1. Primers Used in This Study for Detection of EPEC Strains and β-Lactamase Genes.
Gene | Primer Sequence (5' to 3') | Size of Amplicon, bp | Annealing Temperature, °C |
---|---|---|---|
eae | 229 | 55 | |
F-CTGAACCAGATCGTAACGGC | |||
R-TGATAAGCTGCAGTCGAATCC | |||
bfp | 326 | 58 | |
F-AATGGTGCTTGCGCTTGCTGC | |||
R-GCCGCTTTATCCAACCTGGTA | |||
stx1 | 302 | 55 | |
F-CGCTGAATGTCATTCGCTCTGC | |||
R-CGTGGTATAGCTACTGTCACC | |||
stx2 | 516 | 56 | |
F-CCTCGGTATCCTATTCCCGG | |||
R-CTGCTGTGACAGTGACAAAACGC | |||
blaCTX-M1 | 863 | 54 | |
F-GGTTAAAAAATCACTGCGTC | |||
R-TTGGTGACGATTTTAGCCGC | |||
blaCTX-M2 | 865 | 54 | |
F-ATGATGACTCAGAGCATTCG | |||
R-TGGGTTACGATTTTCGCCGC | |||
blaCTX-M9 | 869 | 54 | |
F-ATGGTGACAAAGAGAGTGCA | |||
R-CCCTTCGGCGATGATTCTC | |||
blaCTX-M15 | 995 | 55 | |
F-CACACGTGGAATTTAGGGACT | |||
R-GCCGTCTAAGGCGATAAACA | |||
SHV | 230 | 56 | |
F-AAGATCCACTATCGCCAGCAG | |||
R-ATTCAGTTCCGTTTCCCAGCGG | |||
bla TEM | 856 | 53 | |
F-ATGAGTATTCAACATTTCCGC | |||
R-CAATGCTTAATCAGTGAGG |