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. 2015 Apr 18;8(4):e15620. doi: 10.5812/jjm.8(4)2015.15620

Table 1. Primers Used in This Study for Detection of EPEC Strains and β-Lactamase Genes.

Gene Primer Sequence (5' to 3') Size of Amplicon, bp Annealing Temperature, °C
eae 229 55
F-CTGAACCAGATCGTAACGGC
R-TGATAAGCTGCAGTCGAATCC
bfp 326 58
F-AATGGTGCTTGCGCTTGCTGC
R-GCCGCTTTATCCAACCTGGTA
stx1 302 55
F-CGCTGAATGTCATTCGCTCTGC
R-CGTGGTATAGCTACTGTCACC
stx2 516 56
F-CCTCGGTATCCTATTCCCGG
R-CTGCTGTGACAGTGACAAAACGC
blaCTX-M1 863 54
F-GGTTAAAAAATCACTGCGTC
R-TTGGTGACGATTTTAGCCGC
blaCTX-M2 865 54
F-ATGATGACTCAGAGCATTCG
R-TGGGTTACGATTTTCGCCGC
blaCTX-M9 869 54
F-ATGGTGACAAAGAGAGTGCA
R-CCCTTCGGCGATGATTCTC
blaCTX-M15 995 55
F-CACACGTGGAATTTAGGGACT
R-GCCGTCTAAGGCGATAAACA
SHV 230 56
F-AAGATCCACTATCGCCAGCAG
R-ATTCAGTTCCGTTTCCCAGCGG
bla TEM 856 53
F-ATGAGTATTCAACATTTCCGC
R-CAATGCTTAATCAGTGAGG