Table 2. List of Locus-Specific PCR Primers Selected for MLVA Assay.
| Loci | Primer Sequence (5' to 3') | Repeat Sizes at Each Locus, bp | Annealing Temperature, °C |
|---|---|---|---|
| ms06 | 39 | 55 | |
| F-AAACGGGAGAGCCGGTTATT | |||
| R-TGTTGGTACAACGGCTCCTG | |||
| ms07 | 39 | 55 | |
| F-GTCAGTTCGCCCAGACACAG | |||
| R-CGGTGTCAGCAAATCCAGAG | |||
| ms09 | 179 | 55 | |
| F-GTGCCATCGGGCAAAATTAG | |||
| R-CCGATAAGGGAGCAGGCTAGT | |||
| ms11 | 96 | 55 | |
| F-GAAACAGGCCCAGGCTACAC | |||
| R-CTGGCGCTGGTTATGGGTAT | |||
| ms21 | 141 | 55 | |
| F-GCTGATGGCGAAGGAGAAGA | |||
| R-GGGAGTATGCGGTCAAAAGC | |||
| ms23 | 375 | 55 | |
| F-GCTCCGCTGATTGACTCCTT | |||
| R-CGGTTGCTCGACCACTAACA | |||
| ms32 | 101 | 55 | |
| F-GAGATTGCCGAAGTGTTGC | |||
| R-AACTGGCGGCGTTTATCAAG |