Table 3. Analysis of Variance for the Effect of the Independent Variables on the Dependent Variables and the Regression Coefficients, of the Fitted Quadratic Equations Obtained Obtained From Experimental Results; Regression Coefficients, R2, and P or Probability Values for Four Dependent Variables for rhIFN-β Production ^a.

Source	DF	rhIFN-β Production, mg L-1				Acetate Excretion, g L-1
		Sum of Squares	Mean Square	F Value	P Value	Sum of Squares	Mean Square	F Value	P Value
Model	9	0.034	3.754E - 003	47.92	0.0003	5.57	0.62	24.57	0.0013
X1	1	9.112E - 003	9.112E - 003	116.33	0.0001	5.06	5.06	200.90	˂ 0.0001
X 2	1	6.125E - 004	6.125E - 004	7.82	0.0382	0.021	0.021	0.83	0.4028
X 3	1	0.013	0.013	163.4	˂ 0.0001	0.011	0.011	0.42	0.5466
X 1 X 2	1	2.025E - 003	2.025E - 003	25.85	0.0038	2.250E - 004	2.250E - 004	8.940E - 003	0.9283
X 1 X 3	1	0.000	0.000	0.000	1.0000	4.225E - 003	4.225E - 003	0.17	0.6990
X 2 X 3	1	1.600E - 003	1.600E - 003	20.43	0.0063	0.084	0.084	3.34	0.1271
X 1 2	1	2.878E - 003	2.878E - 003	36.73	0.0018	0.35	0.35	13.80	0.0138
X 2 2	1	5.308E - 003	5.308E - 003	67.77	0.0004	0.052	0.052	2.08	0.2085
X 3 2	1	1.083E - 004	1.083E - 004	1.38	0.2925	0.023	0.023	0.92	0.3817
Residual	5	3.917E - 004	7.833E - 005			0.13	0.025
Lack of fit	3	3.250E - 004	1.083E - 004	3.25	0.2441	0.13	0.042	1257.75	0.008
Pure error	2	6.667E - 005	3.333E - 005			6.667E - 005	3.333E - 005
Cor. total	14	0.034				5.69
SD		8.851E - 003				0.16
Mean		0.28				1.50
CV, %		3.21				10.60
PRESS		5.350E - 003				2.01
R 2		0.9885				0.9779
R 2 adj		0.9679				0.9381
R 2 pred		0.8434				0.6464
Adeq. precision	23.698					13.452

^a Abbreviations: DF, degree of freedom; PRESS, predicted residual sums of squares; CV, Coefficient of variation.