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. 2015 May 17;2015:426429. doi: 10.1155/2015/426429

Figure 5.

Figure 5

(a) Effect of P4 + PP1 on cell migration of MB231Br in presence of MIF. The up panels of (a) show the wound closure assays of MB231Br and MB231 cells with or without P4 and/or PP1 treatments as indicated. All of the cells were preincubated with MIF. Images were taken under confocal microscope using ×10 objective lens. The lower panel of (a) shows the summarized data from two independent assays (∗0.05 > P > 0.01). (b) Effect of P4 + PP1 on cell migration of MB231Br in presence of anti-PGRMC1 antibody. Growth-arrested MB231Br and MB231 cells were treated with or without P4 and/or PP1 treatment as indicated. Western blot assays for evaluating PGRMC1 expression were performed. The up panel of (b) shows the representative image from three Western blot assays. The middle panel of (b) shows the wound closure assays of MB231Br cells with or without P4 and/or PP1 treatments as indicated. Images were taken under confocal microscope using ×10 objective lens. The graph in the bottom of (b) shows the summarized data from four independent assays (∗0.05 > P > 0.01, ∗∗ P < 0.01).