. 2015 May 17;2015:296721. doi: 10.1155/2015/296721

Table 1.

The polyamine profile: putrescine (Put), spermidine (Spd), spermine (Spm), and total polyamine (Total) in Caco-2, HT-29, and SW480 cell lines following administration of vitamin K1 (VK1) increasing concentrations (from 10 μM to 200 μM) after 24 h, 48 h, and 72 h.

Caco-2		Control	VK1 10 μM	VK1 50 μM	VK1 100 μM	VK1 200 μM
24 h	Put	0.70 ± 0.06	0.34 ± 0.03	0.30 ± 0.05	0.15 ± 0.03^∗	0.13 ± 0.03^∗
	Spd	12.17 ± 0.44	12.30 ± 0.95	9.43 ± 0.35	8.13 ± 1.07^∗	7.93 ± 0.71^∗
	Spm	11.63 ± 0.33	12.07 ± 0.52	10.87 ± 0.29	11.53 ± 0.26	11.33 ± 0.67
	Total	24.50 ± 0.72	24.71 ± 0.39	20.60 ± 0.62	19.82 ± 0.86^∗	19.40 ± 0.70^∗

48 h	Put	0.87 ± 0.07	0.57 ± 0.07	0.33 ± 0.04^∗	0.32 ± 0.03^∗	0.32 ± 0.05^∗
	Spd	16.47 ± 0.33	10.53 ± 0.37	9.63 ± 0.38	8.87 ± 0.29^∗	8.53 ± 0.29^∗
	Spm	13.07 ± 0.69	12.73 ± 0.48	12.13 ± 0.47	12.60 ± 0.49	11.97 ± 0.022
	Total	30.41 ± 0.83	23.57 ± 0.74	21.96 ± 0.70^∗	21.78 ± 0.39^∗	21.15 ± 0.53^∗

72 h	Put	0.57 ± 0.04	0.48 ± 0.05	0.19 ± 0.03^∗	0.24 ± 0.03^∗	0.23 ± 0.03^∗
	Spd	10.53 ± 0.32	9.72 ± 0.40	6.51 ± 0.46^∗	5.89 ± 0.31^∗	5.93 ± 0.48^∗
	Spm	15.83 ± 0.35	14.93 ± 0.46	13.25 ± 0.52^∗	13.37 ± 0.43^∗	13.40 ± 0.38^∗
	Total	26.94 ± 0.70	25.13 ± 0.92	19.95 ± 0.88^∗	19.50 ± 0.67^∗	19.56 ± 0.75^∗

HT-29		Control	VK1 10 μM	VK1 50 μM	VK1 100 μM	VK1 200 μM

24 h	Put	0.76 ± 0.05	0.65 ± 0.04	0.53 ± 0.13	0.55 ± 0.10	0.58 ± 0.08
	Spd	13.04 ± 0.05	12.67 ± 0.18	13.03 ± 0.12	12.93 ± 0.18	12.76 ± 0.23
	Spm	9.70 ± 0.32	9.93 ± 0.28	9.60 ± 0.63	10.13 ± 0.54	9.97 ± 0.24
	Total	23.49 ± 0.39	23.27 ± 0.35	23.30 ± 0.76	23.71 ± 0.55	23.38 ± 0.47

48 h	Put	1.41 ± 0.11	1.14 ± 0.23	1.06 ± 0.18	0.33 ± 0.12^∗	0.22 ± 0.04^∗
	Spd	10.11 ± 0.27	9.43 ± 0.24	9.45 ± 0.19	6.93 ± 0.49^∗	6.25 ± 0.16^∗
	Spm	14.40 ± 0.47	14.47 ± 0.20	13.40 ± 0.40	7.27 ± 0.39^∗	7.13 ± 0.42^∗
	Total	25.92 ± 0.81	25.04 ± 0.55	23.91 ± 0.43	14.53 ± 0.91^∗	13.60 ± 0.54^∗

72 h	Put	0.53 ± 0.18	0.52 ± 0.08	0.30 ± 0.03	0.27 ± 0.04^∗	0.21 ± 0.04^∗
	Spd	8.44 ± 0.29	8.46 ± 0.31	7.50 ± 0.43	7.23 ± 0.34^∗	6.60 ± 0.32^∗
	Spm	13.50 ± 0.32	10.50 ± 0.46	8.30 ± 0.32	6.87 ± 0.38^∗	6.87 ± 0.97^∗
	Total	22.47 ± 0.79	19.48 ± 0.84	16.10 ± 0.66	14.37 ± 0.76^∗	13.68 ± 1.29^∗

SW480		Control	VK1 10 μM	VK1 50 μM	VK1 100 μM	VK1 200 μM

24 h	Put	2.24 ± 0.20	1.45 ± 0.09	1.03 ± 0.14	0.72 ± 0.12^∗	0.78 ± 0.15^∗
	Spd	13.27 ± 0.26	11.74 ± 0.14	9.73 ± 0.32	8.53 ± 0.41^∗	8.24 ± 0.33^∗
	Spm	12.93 ± 0.29	12.03 ± 0.20	10.77 ± 0.29	9.33 ± 0.18^∗	9.22 ± 0.23^∗
	Total	28.44 ± 0.75	25.22 ± 0.43	21.53 ± 0.75	18.59 ± 0.64^∗	18.24 ± 0.50^∗

48 h	Put	1.59 ± 0.17	1.04 ± 0.18	0.79 ± 0.11	0.35 ± 0.06^∗	0.36 ± 0.09^∗
	Spd	9.93 ± 0.29	8.73 ± 0.39	7.60 ± 0.40	6.02 ± 0.36^∗	5.83 ± 0.45^∗
	Spm	13.13 ± 0.40	13.05 ± 0.62	12.16 ± 0.20	11.68 ± 0.29^∗	11.87 ± 0.24^∗
	Total	24.65 ± 0.52	22.82 ± 0.45	20.55 ± 0.72	18.05 ± 0.53^∗	18.06 ± 0.54^∗

72 h	Put	1.79 ± 0.11	1.57 ± 0.14	1.65 ± 0.09	0.93 ± 0.12^∗	0.93 ± 0.13^∗
	Spd	7.31 ± 0.21	6.58 ± 0.25	6.27 ± 0.17	4.91 ± 0.27^∗	4.77 ± 0.22^∗
	Spm	12.58 ± 0.23	12.18 ± 0.18	12.38 ± 0.38	12.03 ± 0.16^∗	11.83 ± 0.20^∗
	Total	21.68 ± 0.55	20.33 ± 0.46	20.30 ± 0.64	17.87 ± 0.44^∗	17.53 ± 0.47^∗

^∗ P < 0.05 compared to control cells.