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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: J Inorg Biochem. 2014 Jul 8;140:245–254. doi: 10.1016/j.jinorgbio.2014.06.018

Figure 5.

Figure 5

Formation of tripyrrole derivatives by the action of HOCl on the active site heme moiety. EPO-HOCl reaction mixture was separated by reverse-phase HPLC and subjected to ESI/MS in the positive ion mode as described under the Materials and Methods section. The major reaction product produced an intense peak at 3.8 min. Examination of the MS spectrum revealed that the [M + H]+ ion had a m/z 516. The extracted ion chromatogram (A) and the MS spectrum of this peak (B) are depicted. See Table 1 for the assigned molecular formula and structure corresponding to this product.