Fig 7. Bub2/Bfa1 and Tem1 asymmetry is important for proper Kar9 distribution and spindle positioning.

A-B: Percentage of metaphase cells carrying Kar9-eGFP (A), Kar9-YFP or Bfa1-eGFP (B) on one SPB (strong asymmetry), both SPBs unequally (partial asymmetry) or both SPBs equally (symmetry) for the indicated strains after formaldehyde fixation. Metaphase and anaphase cells were identified by means of the co-expressed SPB marker Spc42-mCherry. C-D: Spindle position (distance between the nearest SPB and the bud neck) and orientation (angle that the spindle forms with respect to the cell polarity axis) were measured in metaphase spindles of cells with the indicated genotypes after formaldehyde fixation (n≥100).