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. 2015 Feb 6;11(2):e1004629. doi: 10.1371/journal.ppat.1004629

Figure 5. SPIN6 interacts with OsRac1 in vitro and in vivo, and catalyzes OsRac1 hydrolysis in vitro.

Figure 5

(A) SPIN6 interacts with OsRac1 in yeast. The Mav203 yeast transformant diluted 10, 100, or 1000 times was plated on synthetic dextrose medium without Trp, Leu, or His (SD-LTH) and with 0 mM or 40 mM 3-amino-1,2,4,- triazole (3AT). EV-Empty Vector (B) The Co-IP assay of SPIN6 and OsRac1 in N. benthamiana. The GFP:Spin6 and Myc:OsRac1 plasmids were used for co-infiltration in N. benthamiana. (C) BiFC assay of SPIN6 and OsRac1 in N. benthamiana. The Spin6:CeYFP and NeYFP:OsRac1 plasmids were used for co-infiltration. (D) In vitro GAP activity assay of SPIN6. SPIN6 refers to GST-fused SPIN6 protein. OsRac1 refers to MBP-fused OsRac1 protein. GST and MBP are controls. Values are means and standard errors of three replications.