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. Author manuscript; available in PMC: 2015 May 31.
Published in final edited form as: Int J Med Microbiol. 2014 Sep 26;304(8):1218–1225. doi: 10.1016/j.ijmm.2014.09.003

Figure 1.

Figure 1

PCR mapping of regions of mini-transposon plasmid pMT85 (A) present in the genomes of gentamicin resistant selected clonal strains (B). Presence of the gentamicin resistance gene (primers 1518F to 1898R) was only found in transposon mutated strains. PCR probing for different regions of the plasmid identified that strains such as HPA5 and U6 only contain plasmid DNA from between the inverted repeat (IR) regions. Whereas, other transposon mutated strains (HPA56, HPA56 and O10) from different serovars (SV) contain mini-transposon plasmid DNA that include some of the transposase (Tnase) gene. No Tn indicates parental strain, while different numbers after Tn indicate clones from separate experiments. Expected amplicon size is indicated to the right of the figure.