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. Author manuscript; available in PMC: 2016 Jun 15.
Published in final edited form as: Dev Biol. 2015 Apr 11;402(2):229–238. doi: 10.1016/j.ydbio.2015.03.019

Figure 3.

Figure 3

Hair cell and support cell regeneration in adult zebrafish after multiple sequential neomycin treatments. (A) Adult Tg(sqet20Et;alpha-1tubulin:tdTomato) zebrafish were treated with 400 μM neomycin and allowed to recover for 10 sequential neomycin-induced hair cell destruction/regeneration cycles. For each cycle hair cells were allowed to regenerate for 72 hr. We allowed an extra 24hrs of recovery after the 9th neomycin treatment to have hair cells numbers surpass 85% of the previous hair cell quantification. Results are graphed as average number of hair cells per neuromast (± s.d.) for each treatment group. N= 4 adult zebrafish per group, 40+ neuromasts per group. (B) Support cell averages were quantified from adult Tg(pou4f3:gap43-GFP) zebrafish that were neomycin treated 1, 3, 6, and 10 sequential times, euthanized, and stained with the pan-nuclear stain Sytox (green) after each indicated neomycin treatment. Results were graphed as support cell averages per neuromast (±s.d.) for each treatment group. N= 3-4 adult zebrafish per group, 15+ neuromasts per group.