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. Author manuscript; available in PMC: 2016 Jun 15.
Published in final edited form as: Dev Biol. 2015 Apr 11;402(2):229–238. doi: 10.1016/j.ydbio.2015.03.019

Figure 4.

Figure 4

In adult zebrafish, BrdU labeled lateral line hair cells are lost over time under ambient conditions. (A) Schematic illustrating the experimental protocol: fish were treated with 400 μM neomycin for 1 hour, then allowed to recover for 11 hours, followed by a 36 hour 5 mM BrdU incubation period, and finally fixed and stained at the indicated time-points. (B). Representative confocal z-stack maximum intensity projection images of neuromasts with hair cells labeled green with GFP and BrdU positive cell nuclei marked red. BrdU+ hair cells 0 days (left panel), 6 days (middle panel), and 12 days (right panel) after hair cell regeneration. Scale bar, 10 μM (C) Graph illustrating stacked averages (±s.d.) of GFP+ only hair cells (green) and GFP+ hair cells co-labeled with BrdU (red). N= 2-3 adult zebrafish per condition, 30+ neuromasts per group.