Table 2.
Phosphotyrosine-containing proteins identified with 2DGE-based Western blotting and tandem mass spectrometry.
| Spot number | Swiss-Prot number | Protein name | Monoisotopic mass of neutral peptide M r (calc) | Phosphotyrosine-containing peptide sequences | Predicted Tyr-phosphomotif | Tyr-phosphomotif position |
|---|---|---|---|---|---|---|
| 3 | Q13332 | Receptor-type tyrosine-protein phosphatase S | 1940.8 | GY*QVHY*VRMEGAEAR | RtprDenvY Ksq.Dgp.Y Rgv.EgsdY |
97–105 370–376 1728–1735 |
| 42 | Q13332 | Receptor-type tyrosine-protein phosphatase S | 1940.8 | GY*QVHY*VRMEGAEAR | RtprDenvY Ksq.Dgp.Y Rgv.EgsdY |
97–105 370–376 1728–1735 |
| 6 | Q96P48 | Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 | 1496.5 | (RLDTDHLR)Y*FDSNKDAY*SK | RldtDhlrY RchlEak.Y Kyr.Egk.Y Kpl.Ekh.Y |
351–359 637–644 643–649 731–737 |
| 6 | Q8TEP8 | Centrosomal protein of 192 kDa | 1792.8 | KDVLDFGDLTY*GGWK | Kkt.EvsrY | 190–197 |
| 12 | Q9Y4D7 | Plexin-D1 | 2110.0 | (KSRDR)FSY*VLPLVHSLEPTM#GPK | Ksr.DrfsY Kdl.DtekY |
971–978 1666–1673 |
| 21 | Q9P2D3 | HEAT repeat-containing protein 5B | 1325.6 | M#AQY*SFDKLK | RnkdDtaaY Kal.EnsnY ReaaEvceY |
100–108 225–232 1096–1104 |
| 21 | Q5MCW4 | Zinc finger protein 569 | 1511.6 | KEHCEY*NEPVK | KhtgEkp.Y | 655–662 |
| 42 | P06865 | Beta-hexosaminidase subunit alpha | 1187.5 | EDIPVNY*MK | Kql.Esf.Y | 349–355 |
| 46 | P49639 | Homeobox protein Hox-A1 | 1967.9 | TGKVGEY*GY*LGQPNAVR | Kvg.Eyg.Y | 219–225 |
| 47 | Q6P2Q9 | Pre-mRNA-processing-splicing factor 8 | 1325.6 | HTLAY*DKGWR | KfrhDkrvY RvrtDfkqY |
80–88 1437–1445 |
Y* = phosphotyrosine residue, M# = oxidated methine residue.