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. 2015 May 18;2015:830813. doi: 10.1155/2015/830813

Table 1.

Primer sequences.

Genes Primer sequences (5′→3′) PCR product (bp) PCR conditions
cagA ACC CTA GTC GGT AAT GGG 521 94°C, 1 min; 52°C, 1 min; 72°C, 1 min (35 cycles)
GCT TTA GCT TCT GAY ACY GC*

vacA
s1/s2 ATG GAA ATA CAA CAA ACA CAC 259/268 94°C, 1 min; 52°C, 1 min; 72°C, 1 min (35 cycles)
CTG CTT GAA TGC GCC AAA C
m1/m2 CAA TCT GTC CAA TCA AGC GAG 567/642
GCG TCA AAA TAA TTC CAA GG

jhp0562/β-(1,3)galT TGA AAA GCC CTT TTG ATT TTG 301/602 95°C, 30 s; 56°C, 30 s; 72°C, 1 s (35 cycles)
GCT GTA GTG GCC ACA TAC ACG

*Y = C + T.