Table 1.
Genes | Primer sequences (5′→3′) | PCR product (bp) | PCR conditions |
---|---|---|---|
cagA | ACC CTA GTC GGT AAT GGG | 521 | 94°C, 1 min; 52°C, 1 min; 72°C, 1 min (35 cycles) |
GCT TTA GCT TCT GAY ACY GC* | |||
| |||
vacA | |||
s1/s2 | ATG GAA ATA CAA CAA ACA CAC | 259/268 | 94°C, 1 min; 52°C, 1 min; 72°C, 1 min (35 cycles) |
CTG CTT GAA TGC GCC AAA C | |||
m1/m2 | CAA TCT GTC CAA TCA AGC GAG | 567/642 | |
GCG TCA AAA TAA TTC CAA GG | |||
| |||
jhp0562/β-(1,3)galT | TGA AAA GCC CTT TTG ATT TTG | 301/602 | 95°C, 30 s; 56°C, 30 s; 72°C, 1 s (35 cycles) |
GCT GTA GTG GCC ACA TAC ACG |
*Y = C + T.