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. 2015 May 18;4(5):e149. doi: 10.1038/oncsis.2015.12

Figure 2.

Figure 2

Sgt1 heterozygous knockout suppresses Hras-mediated transformation and tumorigenicity of p53−/− MEF cells and increases survival of p53−/− mice. (a) Sgt1+/+ p53−/− and Sgt1+/− p53−/− MEF cells transduced with Hras were used for focus-formation assays. After 2 weeks of incubation, cells were stained and the number of foci was counted. The experiments were repeated three times, and similar results were obtained. Average values±s.d. are shown (∗∗P<0.01; unpaired t test). (b) Hras-transduced MEF cells were injected into immunodeficient CB17SC-F scid−/− female mice. Tumor volumes were measured at indicated time points. Average values±s.d. are shown (∗∗∗∗P<0.0001; unpaired t-test). Mice in the blue curve were killed owing to dehydration on the twenty-first day or the twenty-fourth day. (c) The survival estimates of Sgt1+/+ and Sgt1+/− mice are shown. (d) The survival curve of Sgt1+/+ p53−/− and Sgt1+/− p53−/− mice are shown (P<0.05; log-rank test).