Figure 6.

Progression of sarcolemmal damage after injury. Immunofluorescence labeling of tibialis anterior muscle cross sections reveals that after injury, the number of fibers that are positive for mouse IgG-positive (Ms-IgG⁺) entry (a marker of sarcolemmal damage, arrows) increases slowly over several hours and days in dysferlin-deficient A/J, similar to the appearance of damaged fibers in hematoxylin and eosin–stained sections. Compared with A/J muscle, very few fibers in A/WySnJ muscle are positive for Ms-IgG. Myofiber boundaries are labeled green, with wheat germ agglutinin (WGA) conjugated to Alexa 488 (Life Technologies Corporation, Carlsbad, CA), and nuclei are labeled blue with DAPI.