Figure 7.

Progression of neutrophil and macrophage infiltration after injury. Frozen sections of muscle collected at different times after injury were immunolabeled with antibodies to neutrophils or macrophages. Dystrophin was also labeled to outline the boundaries of healthy myofibers. Low levels of neutrophil infiltration occur in dysferlin-deficient A/J muscle at early times after injury. Macrophage infiltration is more pronounced, however, and becomes very prominent at 48 and 72 hours after large-strain injury. Neutrophils and macrophages are labeled red, and nuclei and myofiber boundaries are labeled blue (DAPI and dystrophin, respectively). Ly6G⁺, positive for neutrophil marker (MCA2387; AbD Serotec, Kidlington, UK).